Research Article | Open Access | 10.31586/Physiology.0302.02

The Effects of Rosemary Extract on Spermatogenesis and Sexual Hormones of Mice under Heat Stress

  • Mehrdad Modaresi1,* and Mansoureh Emadi2
    Department of Physiology, Isfahan [Khorasgan] Branch, Islamic Azad University, Isfahan, Iran
    Department of Biology, Payam e Noor University, Isfahan Center, Isfahan, Iran


Heat stress can affect reproduction potential as an environmental factor. This Study was carried out to investigate the effects of rosemary extract on spermatogenesis and sexual hormones of laboratory mice under heat stress. 50 male mature mice were examined in five groups including a control group and four experimental groups [0, 100, 200 and 400 mg/kg of rosemary extract]. Samples were kept under heat stress four hours a day and received the extract doses for 30 days. At the end of the experiment, the amount of testosterone, LH, and FSH hormones plus the number of spermatogenic cells were measured. Obtained data were analyzed using the SPSS program. Heat stress in zero doses reduced testosterone, LH, and FSH significantly whereas rosemary extract increased testosterone and LH in 200 and 400 doses and FSH in 100,200, and 400 doses. Primary spermatocytes were decreased in zero doses significantly but increased significantly in other experimental groups [p<0.05]. In general, Heat stress reduces male sex hormones and spermatogenic cells but rosemary extract compensated this reduction dose-dependently and improved sexual potential under heat stress.

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Gayton A. 2011. Medical physiology (Translated by Farrukh Shadan), Tehran University. 2, 245.
Collier RJ, Dahl GE, Van Baali MJ. 2006. Major advances associated with environmental effects on dairy cattle. J Dairy Sci. 89, 1244-53.
Premkumar K, Abraham SK, Santhiya ST, Ramesh A. 2003. Protective effects of saffron (Crocus sativus L.) on genotoxin-induced oxidative stress in Swiss albini mice Phytother Research. 17, 614-17.
Modaresi M. 2012. A comparative analysis of the effects of garlic, elderberry and black seed extract on the immune system in mice. J Anim Vet Adv. 11 (4), 458-61.
Ojeda-sana A M, Van baren C M, Elechosa M A, Juarez M A, Morenos S. 2013. New insights into antibacterial and antioxidant activities of rosemary essential oils and their main components. Food Control. 31(1), 189?195.
Posadas S, Caz V, Largo C, De La Gandara B, Matallans B, Reglero G. et al. 2009. Protective effect of supercritical fluid rosemary extract, Rosmarinus officinalis, on antioxidants of major organs of aged rats. Experimental gerontology. 44(6), 383?389.
Modaresi M, Mohajer M. 2015. The Effect of Garlic Extract on Spermatogenesis and Sexual Hormones in Heat-Stressed Male Mice. zumsj. 23 (101), 88-97.
Ghiasi Ghalehkandi J. 2014. Garlic (Allium sativum) juice protects from semen oxidative stress in male rats exposed to chromium chloride. Anim Reprod. 15, 526-32.
Arts M J, Haenen G R, Voss H P, Bast A. 2001. Masking of antioxidant capacity by the interaction of flavonoids with protein. Food and Chemical Toxicology. 39(8), 787?791.
Satoh T, Kosaka K, Itoh K, Kobayashi A, Yamamoto M, Shimojo Y. et al. 2008. Carnosic acid,a catechol?type electrophilic compound, protects neurons both in vitro and in vivo through activation of the Keap1/Nrf2 pathway via S?alkylation of targeted cysteines on Keap1. Journal of neurochemistry. 104(4), 1116?1131.
Satoh T, Izumi M, Inukai Y, Tsutsumi Y, Nakayama N, Kosaka Kosaka. et al. 2008. Carnosic acid protects neuronal HT22 Cells through activation of the antioxidant-responsive element in free carboxylic acid-and catechol hydroxyl moieties-dependent manners. Neuroscience letters. 434(3), 260?265.
Jasim MA, Al-Tahan FJ. 2012. Study of the effect of decorticated and defatted castor seeds (Ricinus communis Linn.) on testosterone level and testicular architecture of male mice. J Tikrit University of Agriculture Sci. 12,176-80.
Kaur R, Kaur K. 2000. Effects of dietary selenium (SE) on morphology of testis and cauda epididymis in rats. Indian J Physiol Pharmacol. 44, 265-72.
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